GSM5884327: LAN233A3; Mus musculus; RNA-Seq - SRA

SRX14121141: GSM5884327: LAN233A3; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 27.7M spots, 5.6G bases, 2.4Gb downloads

External Id: GSM5884327_r1

Submitted by: Francis Crick Institute

Study: Transcriptomic changes in embryonic hearts from Dp3Tyb and Ts1Rhr mouse models of Down Syndrome

PRJNA804914 • SRP359090 • All experiments • All runs

show Abstracthide Abstract

We performed bulk RNAseq on whole hearts from E13.5 Dp3Tyb embryos and from wild-type littermates, as well as hearts from E13.5 Ts1Rhr embryos and wild-type littermates. Analysis showed the expected increased expression of the Hsa21-orthologous genes on Mmu16 that are present in 3 copies. Gene set enrichment analysis identified pathways that are altered in Dp3Tyb and Ts1Rhr hearts. Overall design: RNAseq on 5 E13.5 Dp3Tyb embryonic hearts and 5 wild-type littermate embryonic hearts, and on 5 E13.5 Ts1Rhr embryonic hearts and 5 wild-type littermate embryonic hearts.

Sample: LAN233A3

SAMN25816380 • SRS11943972 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM5884327

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Hearts were dissected and snap frozen and kept at -80C. Samples were lysed (buffer RLT Qiagen RNeasy Minikit) and homogenised using a battery-operated pestle motor mixer followed by RNA extraction using Qiagen RNeasy Mini kit using the standard protocol. cDNA library synthesis using KAPA mRNA HyperPrep Kit (Illumina) and libraries were sequenced on the Illumina HiSeq 4000 following manufacturer's protocols.

Runs: 1 run, 27.7M spots, 5.6G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR17964797	27,658,153	5.6G	2.4Gb	2024-01-26

ID:: 19895086

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity