GSM5884315: LAN757A11; Homo sapiens; RNA-Seq - SRA

SRX14121129: GSM5884315: LAN757A11; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 26M spots, 2.6G bases, 1.1Gb downloads

External Id: GSM5884315_r1

Submitted by: Francis Crick Institute

Study: Transcriptomic analysis of human foetal hearts with or without Down Syndrome

PRJNA804915 • SRP359089 • All experiments • All runs

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We performed bulk RNAseq on whole hearts from foetuses with Down syndrome and age-matched, sex-matched euploid controls. Analysis showed the expected increased expression of the genes on Hsa21 that are present in 3 copies. Gene set enrichment analysis identified pathways that are altered in DS hearts. Overall design: RNAseq on 5 human embryonic hearts with Down syndrome and on 5 euploid age- and sex-matched control hearts.

Sample: LAN757A11

SAMN25816058 • SRS11943960 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM5884315

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: samples were obtained and snap frozen and kept at -80C. Samples were lysed (buffer RLT Qiagen Rneasy Maxi kit) and homogenised using Macs Milteni M tubes and gentleMACS dissociator followed by RNA extraction using Qiagen Rneasy Maxi kit following the standard protocol

Runs: 1 run, 26M spots, 2.6G bases, 1.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR17964779	25,950,729	2.6G	1.1Gb	2024-01-26

ID:: 19895074

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Sequence Read Archive

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