GSM5731243: FOXA2 WT, Mature Hepatocytes, Samp9; Homo sapiens; RNA-Seq - SRA

SRX13396874: GSM5731243: FOXA2 WT, Mature Hepatocytes, Samp9; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 25.3M spots, 3.8G bases, 1.5Gb downloads

Submitted by: NCBI (GEO)

Study: Effect of FOXA2 knockout (KO) on transcriptome wide gene expression in hepatic progenitors (HP) and mature hepatocytes (MH)

PRJNA788448 • SRP350523 • All experiments • All runs

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iPSC generated from healthy controls and FOXA2 knockout (KO) were allowed to undergo hepatic differentiation, and RNA was collected from hepatic progenitors (DAY 10) and mature hepatocytes (DAY21) for next-generation sequencing. Overall design: Transcriptomic profile of FOXA2 KO iPSCs - derived progenitors and mature hepatocytes was compared with healthy control from two biological replicates.

Sample: FOXA2 WT, Mature Hepatocytes, Samp9

SAMN23971340 • SRS11300036 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Cells were collected using TRIzol reagent (Cat Number.15596026) and RNA was extracted using Norgen rna extraction kit (Cat Number. 47700) RNA libraries were prepared for sequencing using TruSeq Stranded mRNA Library(Cat # 20020594, for Hepatic Progenitors) and NEBNext Ultra Directional Library Prep Kit (NEB, E7420L, for Mature Hepatocytes) following the manufacturer's protocol

Experiment attributes:

GEO Accession: GSM5731243

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 25.3M spots, 3.8G bases, 1.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR17217115	25,272,502	3.8G	1.5Gb	2022-08-15

ID:: 18481711

SRA

Sequence Read Archive

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