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SRX13396869: GSM5731238: FOXA2 WT, Hepatic Progenitor, HP4; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 30.8M spots, 4.7G bases, 1.7Gb downloads

Submitted by: NCBI (GEO)
Study: Effect of FOXA2 knockout (KO) on transcriptome wide gene expression in hepatic progenitors (HP) and mature hepatocytes (MH)
PRJNA788448 • SRP350523 • All experiments • All runs
show Abstracthide Abstract
iPSC generated from healthy controls and FOXA2 knockout (KO) were allowed to undergo hepatic differentiation, and RNA was collected from hepatic progenitors (DAY 10) and mature hepatocytes (DAY21) for next-generation sequencing. Overall design: Transcriptomic profile of FOXA2 KO iPSCs - derived progenitors and mature hepatocytes was compared with healthy control from two biological replicates.
Sample: FOXA2 WT, Hepatic Progenitor, HP4
SAMN23971335 • SRS11300030 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Cells were collected using TRIzol reagent (Cat Number.15596026) and RNA was extracted using Norgen rna extraction kit (Cat Number. 47700) RNA libraries were prepared for sequencing using TruSeq Stranded mRNA Library(Cat # 20020594, for Hepatic Progenitors) and NEBNext Ultra Directional Library Prep Kit (NEB, E7420L, for Mature Hepatocytes) following the manufacturer's protocol
Experiment attributes:
GEO Accession: GSM5731238
Links:
Runs: 1 run, 30.8M spots, 4.7G bases, 1.7Gb
Run# of Spots# of BasesSizePublished
SRR1721711030,791,4984.7G1.7Gb2022-08-15

ID:
18481706

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