GSM5723805: Experiment 1_T1000_DMSO_1; Homo sapiens; RNA-Seq - SRA

SRX13351547: GSM5723805: Experiment 1_T1000_DMSO_1; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 48.5M spots, 9.7G bases, 3.6Gb downloads

Submitted by: NCBI (GEO)

Study: Signalling inhibition by ponatinib disrupts alternative lengthening of telomeres (ALT)

PRJNA787207 • SRP349796 • All experiments • All runs

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Analyses of gene expression changes in cells treated with ponatinib (experiment 1), and in cells either lacking JUN or overexpressing JUN (experiment 2). Overall design: Experiment 1: RNA-seq on SAOS-2 or T1000 cells treated with either DMSO (as a control) or ponatinib (250nM for 24 hours); 2 replicates per condition. Experiment 2: Identification of potential JUN transcription targets by RNA-seq in SAOS2 cells lacking JUN (cjun sg vs. LacZ sg as a control) or overexpressing JUN (JUN vs. CTRL (GFP overexpressing cells)); 3 replicates per condition

Sample: Experiment 1_T1000_DMSO_1

SAMN23778190 • SRS11256043 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was extracted using Qiagen RNeasy Mini kit (experiment 1) or Promega Reliaprep RNA cell miniprep kit (experiment 2). Poly-A mRNAs enrichment

Experiment attributes:

GEO Accession: GSM5723805

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 48.5M spots, 9.7G bases, 3.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR17167666	48,460,845	9.7G	3.6Gb	2023-01-30

ID:: 18347490

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Sequence Read Archive

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