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SRX128117: GSM889440: mule3669_day33_CG_RNAseq; Equus asinus x caballus; RNA-Seq
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 23.4M spots, 2G bases, 1.1Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Profiling of differential allelic expression in horse, donkey, mule, and hinny placental tissues.
show Abstracthide Abstract
In eutherian mammals, dosage compensation of X-linked genes is achieved by X chromosome inactivation. X inactivation is random in embryonic and adult tissues, but imprinted X inactivation (paternal X silencing) has been identified in the extraembryonic membranes of the mouse, rat, and cow. Few other species have been studied for this trait, and the data from studies of the human placenta have been discordant or inconclusive. Here, we quantify X inactivation using RNA sequencing of placental tissue from reciprocal hybrids of horse and donkey (mule and hinny). In placental tissue from the equid hybrids and the horse parent the allelic expression pattern was consistent with random X inactivation, and imprinted X inactivation can clearly be excluded. We characterized horse and donkey XIST gene, and demonstrated that XIST allelic expression in female hybrid placental and fetal tissues is negatively correlated with the other X-linked genes chromosome-wide, which is consistent with the XIST-mediated mechanism of X inactivation discovered previously in mice. As the most structurally and morphologically diverse organ in mammals, the placenta also appears to show diverse mechanisms for dosage compensation that may result in differences in conceptus development across species. Overall design: Examine allelic expression from individual samples of invasive trophoblast tissue of the chorionic girdle from gestation day 33-34 conceptuses of 5 horses, 3 donkeys, 6 mules, and 1 hinny.
Sample: mule3669_day33_CG_RNAseq
SAMN00809372 • SRS299252 • All experiments • All runs
Library:
Name: GSM889440: mule3669_day33_CG_RNAseq
Instrument: Illumina Genome Analyzer IIx
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Spot descriptor:
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Experiment attributes:
GEO Accession: GSM889440
Links:
External link:
Runs: 1 run, 23.4M spots, 2G bases, 1.1Gb
Run# of Spots# of BasesSizePublished
SRR44174123,384,3842G1.1Gb2012-05-30

ID:
146167

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