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SRX12755584: GSM5652754: Leaf, high phytotoxic potential, A1; Solanum tuberosum; RNA-Seq
1 BGISEQ (BGISEQ-500) run: 57.8M spots, 5.8G bases, 3.3Gb downloads

Submitted by: NCBI (GEO)
Study: Involvement of flavonol synthase/flavanone 3-hydroxylase-like in expression of phytotoxic potential of potato (Solanum sp.)
show Abstracthide Abstract
Glycoalkaloids are Solanum bioactive compounds and are involved in allelopathic interactions. To achieve a better understanding of plant–plant interactions, it is essential to deeply examine the trait distribution in a segregating population. In the present paper, we used transcriptomic and metabolomic approaches to recognize the phytotoxic abilities of potato plants originating from a diploid segregating F1 population with particular emphasis on glycoalkaloids in potato groups characterized by a high glycoalkaloid content. In potato F1 individuals, six glycoalkaloids were recognized: solasonine, solamargine, a-solanine, a-chaconine, leptinine I, and leptine II. In the bulk samples characterized by a high total glycoalkaloid content and various phytotoxic potential, high - A', low - B' and hormesis - F', a significant role of the glycoalkaloid composition in the expression of phytotoxic potential was revealed. In particular, leptine II, solasonine and solamargine were present. Based on the RNA-seq analysis of the bulk samples, a flavonol synthase/flavanone 3-hydroxylase-like gene responsible for flavonoid synthesis was upregulated in comparison A' vs. B' and A' vs. F' (Log2FC=8.30 and 6.40, respectively). The population-level evaluation of phytotoxic potential confirmed a significant negative correlation between total glycoalkaloid content and phytotoxic potential (r=-0.211) but only after correction for total flavonoid content. Overall design: Examination of potato phytotoxic potential under high glycoalkaloids content.
Sample: Leaf, high phytotoxic potential, A1
SAMN22549361 • SRS10703218 • All experiments • All runs
Library:
Instrument: BGISEQ-500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was isolated using TRIZol method as described Chomczyński and Sacchi (1987). The mRNA was isolated using Dynabeads®mRNA Purification Kit for mRNA enrichment (Ambion, 61006), and cDNA libraries were prepared using using MGIEasy RNA Directional Library Prep Set (MGI, 1000006386). The established cDNA libraries were sequenced on the BGISEQ-500 sequencing platform (BGI Genomics, China) to generate 100-bp paired-end reads (PE100).
Experiment attributes:
GEO Accession: GSM5652754
Links:
Runs: 1 run, 57.8M spots, 5.8G bases, 3.3Gb
Run# of Spots# of BasesSizePublished
SRR1655339857,785,5045.8G3.3Gb2021-10-28

ID:
17356497

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