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SRX12693842: GSM5639494: Mouse; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 325.9M spots, 98.4G bases, 26.9Gb downloads

External Id: GSM5639494_r1
Submitted by: Xian Jiaotong University
Study: Single-cell transcriptomic atlas reveals the evolution of immune cells and molecules across multiple vertebrate species
show Abstracthide Abstract
We utilized single-cell RNA sequencing (scRNA-seq) to carry out comparative transcriptome analysis in various immune cells from the spleens across seven vertebrate species. Overall design: High-dimensional single-cell RNA sequencing (10X Genomics) was used to profile the transcriptomes of total immune cells isolated from seven organism spleens.
Sample: Mouse
SAMN22415780 • SRS10644724 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM5639494
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Spleen tissues were processed with the flat end of a syringe in a 100 mm culture dish containing 5 ml cold FACS buffer (2% FBS in PBS), then passed through a 70 μm cell strainer into a 15 ml tube. Cells were centrifuged to remove the supernatant. Cell pellets were treated with 1ml ACK (Ammonium-Chloride-Potassium) Lysing Buffer to remove the red blood cells. After washing with 10 ml cold FACS buffer, the remaining cells were stained with 7AAD (Part 76332; Lot B226294 Biolegend) for 30 min at 4 °C, then washed and resuspended in cold FACS buffer for flow cytometric sorting using FACS Aria II Cell Sorter (BD Biosciences). Sorted 7AAD- cells with a viability higher than 90% were used for 10X genomics scRNA-seq. Finally, libraries were sequenced on a NovaSeq 6000 system with NovaSeq 6000 S4 Reagent Kit (300 cycles). RNA libraries were prepared for sequencing using standard Illumina protocols
Runs: 1 run, 325.9M spots, 98.4G bases, 26.9Gb
Run# of Spots# of BasesSizePublished
SRR16490735325,923,59798.4G26.9Gb2021-10-21

ID:
17261514

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