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SRX12405093: GSM5603745: PBMCs-8 wpt; Macaca mulatta; RNA-Seq
1 ILLUMINA (HiSeq X Ten) run: 416.6M spots, 125.8G bases, 50.8Gb downloads

Submitted by: NCBI (GEO)
Study: Human Pluripotent Stem Cell-derived Islets Ameliorate Diabetes in Nonhuman Primates [monkey_singlecell]
PRJNA767422 • SRP339355 • All experiments • All runs
show Abstracthide Abstract
Human pluripotent stem cell-derived islets (hPSC-islets) are a promising cell resource for diabetes treatment. Here, we demonstrate that transplantation of human pluripotent stem cell-derived islets into diabetic nonhuman primates effectively restored endogenous insulin secretion and improved glycemic control. Single-cell RNA sequencing analysis of S6D2 clusters confirmed the existence of the three major pancreatic endocrine cell populations (ß cells, a-like cells and d-like cells) and their proportions, which altogether accounted for 80%. Importantly, hierarchical clustering of S6D2 hCiPSC-islets, 10 wpt kidney grafts and primary human islets showed that the hCiPSC differentiated pancreatic endocrine cells shared similar global gene expression profiles to their native counterparts in primary human islets. Single-cell RNA sequencing analysis on PBMCs revealed the potential immune response of recipient macaque to hCiPSC-islets. Overall design: Single-cell RNA sequencing analysis on peripheral blood mononuclear cells (PBMCs) to analyze the potential immune response of recipient macaque to hCiPSC-islets.
Sample: PBMCs-8 wpt
SAMN21903842 • SRS10374275 • All experiments • All runs
Organism: Macaca mulatta
Library:
Instrument: HiSeq X Ten
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Cells were harvested and resuspended in PBS with 0.04% BSA at 1×10^6 cells per milliliter. Then, cell suspensions were loaded on a Chromium Single Cell Controller (10x Genomics) to generate single-cell gel beads in emulsion (GEMs) by using ChromiumTM Single Cell 5' Library & Gel Bead Kit V1 (10x Genomics, 1000006). Captured cells were lysed and the released RNA were barcoded through reverse transcription in individual GEMs. Barcoded cDNAs were pooled and underwent cleanup by using DynaBeads MyOne Silane Beads (Invitrogen, 37002D) and then amplified and cleanup for further next generation library construction. Single-cell RNA seq libraries were prepared using ChromiumTM Single Cell 5' Library Construction Kit V1 (10x Genomics, 1000020) following instructions provided by the manufacturer. Sequencing was performed on an Illumina HiSeq X Ten (Illumina, San Diego, CA, USA) with pair end 150bp. Single-cell RNA sequencing
Experiment attributes:
GEO Accession: GSM5603745
Links:
Runs: 1 run, 416.6M spots, 125.8G bases, 50.8Gb
Run# of Spots# of BasesSizePublished
SRR16119608416,607,203125.8G50.8Gb2022-02-15

ID:
16798856

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  • SRX12405093 (1)
    SRA
  • ATPase family AAA domain-containing protein 5 [Homo sapiens]
    ATPase family AAA domain-containing protein 5 [Homo sapiens]
    gi|26080431|ref|NP_079133.3|
    Protein
  • RecName: Full=CCR4-NOT transcription complex subunit 4; AltName: Full=CCR4-assoc...
    RecName: Full=CCR4-NOT transcription complex subunit 4; AltName: Full=CCR4-associated factor 4; AltName: Full=E3 ubiquitin-protein ligase CNOT4; AltName: Full=Potential transcriptional repressor NOT4Hp; AltName: Full=RING-type E3 ubiquitin transferase CNOT4
    gi|115502372|sp|O95628.3|CNOT4_HUMA
    Protein
  • Echocardiography
    Echocardiography
    Ultrasonic recording of the size, motion, and composition of the heart and surrounding tissues. The standard approach is transthoracic.<br/>Year introduced: 1973(1967)
    MeSH
  • Incidental Findings
    Incidental Findings
    Unanticipated information discovered in the course of testing or medical care. Used in discussions of information that may have social or psychological consequences, such as w...<br/>Year introduced: 2003
    MeSH

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