GSM5603059: ciTotiSC RRBS-seq rep1; Mus musculus; Bisulfite-Seq - SRA

SRX12403369: GSM5603059: ciTotiSC RRBS-seq rep1; Mus musculus; Bisulfite-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 30.7M spots, 9.2G bases, 3Gb downloads

Submitted by: NCBI (GEO)

Study: Induction of mouse totipotent stem cells by a defined chemical cocktail [RRBS]

PRJNA767358 • SRP339302 • All experiments • All runs

show Abstracthide Abstract

We demonstrate induction and long-term maintenance of totipotent stem cells (TotiSCs) from mouse pluripotent stem cells (PSCs) by a combination of three small molecules, TTNPB, 1-Azakenpaullone, and WS6. These cells, which we designated as ciTotiSCs (chemically induced totipotent stem cells), resembled mouse totipotent 2C-embryo stage cells at both transcriptome and epigenome level. Overall design: RRBS-seq of ciTotiSC with two replicates.

Sample: ciTotiSC RRBS-seq rep1

SAMN21899211 • SRS10372578 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina NovaSeq 6000

Strategy: Bisulfite-Seq

Source: GENOMIC

Selection: Reduced Representation

Layout: PAIRED

Construction protocol: Genomic DNAs were extracted using DNeasy Blood and Tissue Kits (QIAGEN) and digested by MspI (NEB). The digested DNAs were filled in and tailed. Then, bisulfate conversion was performed using MethyCode bisulfite conversion kit (Invitrogen). After amplification and purification，all libraries were constructed and subjected to 150 bp paired-ends Illumina Novaseq 6000 platform.

Experiment attributes:

GEO Accession: GSM5603059

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 30.7M spots, 9.2G bases, 3Gb

Run	# of Spots	# of Bases	Size	Published
SRR16117868	30,721,658	9.2G	3Gb	2022-05-26

ID:: 16786968

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