GSM5603034: P1 ciTotiSC RNA-seq rep2; Mus musculus; RNA-Seq - SRA

SRX12403358: GSM5603034: P1 ciTotiSC RNA-seq rep2; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 22.2M spots, 6.7G bases, 1.9Gb downloads

Submitted by: NCBI (GEO)

Study: Induction of mouse totipotent stem cells by a defined chemical cocktail [bulk RNA-seq]

PRJNA767355 • SRP339300 • All experiments • All runs

show Abstracthide Abstract

We demonstrate induction and long-term maintenance of totipotent stem cells (TotiSCs) from mouse pluripotent stem cells (PSCs) by a combination of three small molecules, TTNPB, 1-Azakenpaullone, and WS6. These cells, which we designated as ciTotiSCs (chemically induced totipotent stem cells), resembled mouse totipotent 2C-embryo stage cells at both transcriptome and epigenome level. Overall design: Bulk RNA-seq of P1, P2, P4, P8 ciTotiSC and OG2 mESC. Each has two replicates.

Sample: P1 ciTotiSC RNA-seq rep2

SAMN21898846 • SRS10372567 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNAs were extracted using TRIzol reagent. mRNA-seq libraries were generated using KAPA stranded mRNA library preparation kit (KAPA Biosystems, KK8421) according to the manufacturer's protocol.

Experiment attributes:

GEO Accession: GSM5603034

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 22.2M spots, 6.7G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR16117857	22,227,557	6.7G	1.9Gb	2022-05-26

ID:: 16786940

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