SRA

Abscisic acid (ABA) plays crucial regulatory roles in cold acclimation and deacclimation of grapevine, making it a potential tool to be utilized in vineyards for the acquisition of preferred phenotypes in winter and spring. To understand the function of ABA , we conducted two experiments during cold acclimation and deacclimation and evaluated the impact of exogenous abscisic acid (ABA) on the grapevine transcriptome. RNA-seq data were collected periodically hours or days after ABA treatment. Transcriptomic data were analyzed using principal component analysis (PCA) , hierarchical clustering, unsupervised weighed gene co-expression network analysis (WGCNA), contrast-based differentially expressed genes (DEGs) identification and pre-ranked gene set enrichment analysis (GSEA). Our results suggest that ABA functions differently during cold acclimation and deacclimation by selectively regulating key pathways including auxin/indole acetic acid (IAA) metabolism, galactose metabolism and ribosome biogenesis. We also identified the activation of several apparent negative feedback systems that regulated ABA-induced transcriptomic changes, suggesting the existence of a balancing system in response to excessive ABA. Overall design: In the acclimation experiment, 40 leaf samples were analyzed in a time serie. These samples include eight replicates of control samples at pre-treatment and four replicates of control and ABA-treated samples at 2 h, 4 h, 24 h and 48 h post-treatment. In the deacclimation experiment, 34 bud samples were analyzed in a time serie. These samples include three replicates of control samples at pre-treatment and three replicates of control and ABA-treated samples at 6 h, 12 h, 24 h, 48 h (one more control sample at 48 h) and 72 h post-treatment.