GSM5527483: H2Bp53DKO 3; Mus musculus; RNA-Seq - SRA

SRX11809418: GSM5527483: H2Bp53DKO 3; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 20.6M spots, 1.1G bases, 404.5Mb downloads

Submitted by: NCBI (GEO)

Study: Epithelial XBP1 coordinates TP53-driven DNA damage responses and suppression of intestinal carcinogenesis

PRJNA755990 • SRP333084 • All experiments • All runs

show Abstracthide Abstract

We assessed the impact of intestinal epithelial XBP1 in coordinating epithelial DNA damage responses. As our data revealed that low XBP1 activity in the context of chronic DNA damage is associated with both reduced p53 pathway activity and formation of tumors with metastaic potential in-vivo, we performed RNA sequencing of intestinal organoids (H2b/Xbp1fl/fl, H2b?IEC, H2b/Xbp1?IEC, H2b/p53?IEC) to identify a transcriptional program downstream of p53 that drives the tumorigenic in-vivo phenotype. Overall design: Examination of Differentially Expressed Genes between small intestinal organoids derived from healthy tissue of 8-12 weeks old mice (n = 3) of indicated genotypes.

Sample: H2Bp53DKO 3

SAMN20848388 • SRS9824154 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Organoids were washed in PBS and RNA was extracted following the RNeasy Kit (Qiagen) according to manufacturer's protocol. RNA libraries were prepared using TruSeq stranded mRNA Kit (Illumina) according to manufacturer's instructions. All samples were sequenced using an Illumina HiSeq 4000 with single-end sequencing of 50bp reads

Experiment attributes:

GEO Accession: GSM5527483

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 20.6M spots, 1.1G bases, 404.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR15510523	20,637,891	1.1G	404.5Mb	2021-09-02

ID:: 15726353

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity