GSM1849920: Bcell-pro-R3; Mus musculus; RNA-Seq - SRA

SRX1143985: GSM1849920: Bcell-pro-R3; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 53M spots, 10.6G bases, 5.2Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Long non-coding RNAs in B cells (RNA-Seq)

PRJNA292726 • SRP062350 • All experiments • All runs

show Abstracthide Abstract

Using RNA sequencing and de novo transcript assembly, we identified 4516 lncRNAs expressed in 8 different stages of B cell development and activation. Chromatin immuno-precipitation sequencing was used to classify a substantial fraction (38%) of these lncRNAs as enhancer-associated or promoter-associated RNAs (eRNAs or pRNAs). Overall design: A catalogue of lncRNAs expressed in eight murine B cell populations

Sample: Bcell-pro-R3

SAMN03983978 • SRS1030113 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Female mouse C57BL/6 B cell populations were sorted on BD FACSAriaII, BD Influx or Beckman Coulter MoFlo XDP cell sorters; collected cells were centrifuged and ressuspended in Trizol and RNA was extracted with the Rneasy kit (Qiagen) according to the manufacturer's instructions Libraries were prepared with Illumina's TruSeq stranded mRNA Sample Preparation kit according to the manufacturer's instructions

Experiment attributes:

GEO Accession: GSM1849920

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 53M spots, 10.6G bases, 5.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR2157270	53,042,672	10.6G	5.2Gb	2016-07-18

ID:: 1666272

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