Instrument: Illumina HiSeq 2000
Strategy: RIP-Seq
Source: TRANSCRIPTOMIC
Selection: other
Layout: PAIRED
Construction protocol: For Lsm1 knockdown zyogtes, RNA was harvested using Trizol reagent. For RIP-seq zygotes, Protein-RNA complex were isolated by ANTI-FLAG® M2 Affinity Gel, then RNA was harvested using Trizol reagent. For Lsm1 knockdown zyogtes, RNA libraries were prepared for sequencing using KAPA Stranded RNA-Seq Kit with RiboErase (HMR) (KapaBiosystems, Cat. Nos.KK8483); For RIP-seq zygotes, RNA libraries were prepared for sequencing using SMARTer Stranded Total RNA-Seq Kit v2 (Takara, Cat. Nos. 635005)