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SRX1135955: GSM1846038: LB(HER2+)_0_RNA-Seq; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina Genome Analyzer II) run: 43.2M spots, 10.7G bases, 4.5Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Next Generation Sequencing Transcriptomes of breast cancer
show Abstracthide Abstract
We performed high throughput transcriptome of breast cancer and normal tissues, identifying lincRNA associated molecular subtype with powerful capacity to distinct breast cancer population and predict prognosis. We also identified subtype-specific lincRNAs that may be a useful complement to intrinsic molecular subtype classification when divergence emerges among pathologists.Paired-end transcriptome sequencing were carried out on a cohort of 33 breast tissues from 11 groups including five breast cancer subtypes including luminal A (LA), luminal B (HER2 negative)(LB, HER2-), luminal B (HER2 positive) (LB, HER2+), HER2 and tripple negative breast cancer (TNB), adjacent noncancerous breast tissue (ANT, three samples for each subtype) and the complete normal breast tissues (three samples) Overall design: Examination of transcriptomes in 15 breast cancer and 18 normal tissues.
Sample: LB(HER2+)_0_RNA-Seq
SAMN03979275 • SRS1028595 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina Genome Analyzer II
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was harvested using Trizol reagent, Ribo-Zero™ Gold Kits(Human). Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM1846038
Links:
Runs: 1 run, 43.2M spots, 10.7G bases, 4.5Gb
Run# of Spots# of BasesSizePublished
SRR214825343,203,95010.7G4.5Gb2017-09-07

ID:
1657092

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