GSM5404496: Input_L776; Human immunodeficiency virus 1; OTHER - SRA

SRX11243578: GSM5404496: Input_L776; Human immunodeficiency virus 1; OTHER
1 ILLUMINA (Illumina MiSeq) run: 11,304 spots, 3.4M bases, 2.2Mb downloads

Submitted by: NCBI (GEO)

Study: Functional and Structural Segregation of Overlapping Helices in HIV-1

PRJNA742013 • SRP325862 • All experiments • All runs

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We report deep mutational scanning data for the Env protein's LLP-2 domain in the NL4-3 strain HIV-1 Env. Processed Data repersents counts for each amino acid pre and post spread Overall design: Each fastq file represents either a pre or post-selection virus in which a single codon of the Env LLP-2 has been randomized to NNN. Pre-selection (Inputs) are roughly randomly spread out amongst all 64 codons, while post selection viruses represent only amino acids that are viable in a spreading assay.

Sample: Input_L776

SAMN19918518 • SRS9289534 • All experiments • All runs

Organism: Human immunodeficiency virus 1

Library:

Instrument: Illumina MiSeq

Strategy: OTHER

Source: GENOMIC

Selection: other

Layout: PAIRED

Construction protocol: RNA was extracted from virions by ZR-96 Viral RNA extraction kits (Zymo) and eluted in 15 ul water. cDNA was amplified from 5uL of extracted RNA using the iScript Advanced (BioRad) cDNA synthesis. PCR amplicons were made from DNA using primers specific to the Env LLP-2 region

Experiment attributes:

GEO Accession: GSM5404496

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 11,304 spots, 3.4M bases, 2.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR14930817	11,304	3.4M	2.2Mb	2021-07-17

ID:: 15012372

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