Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted from the liver and muscle after homogenizing the tissues in Trizol reagent (Life Technologies, Carlsbad, CA) following the manufacturer's recommendations. Any residual contaminating DNA was removed by binding the RNA in a RNeasy binding column, treating the column with RNAse free DNAse (Qiagen, Germantown, MD) and eluting the RNA in nuclease free water. The quality of purified RNA including the purity and RNA integrity were evaluated using Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA) at the University of Michigan Advanced Genomics Core. library preparation was carried out using TruSeq RNA Library Prep Kit (Illumina, San Diego, CA)