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SRX11196146: GSM5395538: Single 2 cell embryo. Sample W40 Condition: NT; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 4.8M spots, 704.6M bases, 267.2Mb downloads

Submitted by: NCBI (GEO)
Study: Zygotic genome activation by the totipotency pioneer factor Nr5a2 III
show Abstracthide Abstract
Life begins with a switch in genetic control from the maternal to the embryonic genome during zygotic genome activation (ZGA). Despite its importance, the essential regulators of ZGA remain largely unknown in mammals. Based on de novo motif searches, we identified the orphan nuclear receptor Nr5a2 as a key activator of major ZGA in mouse 2-cell embryos. Nr5a2 is required for progression beyond the 2-cell stage. It binds to its motif within SINE B1/Alu retrotransposable elements found in cis-regulatory regions of ZGA genes. Chemical inhibition suggests that 72% of ZGA genes are regulated by Nr5a2 and potentially other orphan nuclear receptors. Nr5a2 promotes chromatin accessibility during ZGA and binds nucleosomal DNA in vitro. We conclude that Nr5a2 is an essential pioneer factor that regulates ZGA. Overall design: Single embryo RNA seq of SR1848 treated (80) and DMSO control (56) 2C embryos.
Sample: Single 2 cell embryo. Sample W40 Condition: NT
SAMN19812855 • SRS9251581 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Single embryo SmartSeq2 protocol with oligo dT priming RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM5395538
Links:
Runs: 1 run, 4.8M spots, 704.6M bases, 267.2Mb
Run# of Spots# of BasesSizePublished
SRR148790334,760,751704.6M267.2Mb2022-11-25

ID:
14935415

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