GSM5394147: B_1000_p3; Oryzias latipes; RNA-Seq - SRA

SRX11189255: GSM5394147: B_1000_p3; Oryzias latipes; RNA-Seq
1 ILLUMINA (HiSeq X Ten) run: 4.3M spots, 1.3G bases, 492.8Mb downloads

Submitted by: NCBI (GEO)

Study: Effects of Potassium Perchlorate on Developing Medaka Primordial Germ Cells

PRJNA739694 • SRP324878 • All experiments • All runs

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The present study examined the effects of 0, 10, 100, and 1000 µg/L potassium perchlorate exposure on PGCs of medaka embryos and the expression pattern of genes that maintain PGCs integrity and epigenetic processes. Overall design: To investigate the effect of potassium perchlorate on medaka development , fertilized eggs were exposed to 0, 10, 100, and 1000 µg/L of potassium perchlorate for 15 days and developmental defects were examined. Three biological replicates for each treatment

Sample: B_1000_p3

SAMN19798120 • SRS9245477 • All experiments • All runs

Organism: Oryzias latipes

Library:

Instrument: HiSeq X Ten

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Primordial germ cells (PGCs) were isolated according to the method developed by Wang and Bhandari (2020). Genomic DNA and total RNA were extracted using ZR-Duet™ DNA/RNA MiniPrep kit (Zymo Research, Irvine, U.S.A.). RNA sequencing libraries were prepared with NEBNext® UltraTM II RNA Library Prep Kit for Illumina accordingly to the manufacturer's instructional manual. For each sample, 500ng total RNA was used. RNAseq libraries were sequenced on the Illumina platform using the 150 bp pair-end sequencing strategy.

Experiment attributes:

GEO Accession: GSM5394147

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 4.3M spots, 1.3G bases, 492.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR14871791	4,271,782	1.3G	492.8Mb	2024-06-21

ID:: 14918591

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