GSM1828772: HET_CD4_1; Mus musculus; RNA-Seq - SRA

SRX1114423: GSM1828772: HET_CD4_1; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 27.9M spots, 5.6G bases, 3Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Splenic bridging channel dendritic cells survey red blood cells for missing self-CD47

PRJNA290485 • SRP061381 • All experiments • All runs

show Abstracthide Abstract

In vivo exposure of mouse CD4+ splenic DCs to sheep red blood cells causes prompt induction of multiple genes associated with DC activation. Overall design: mRNA profiles from CD4 dendritic cells (sorted from mice immunized with either sheep red blood cells or PBS) were generated using an Illumia HiSeq 2500. Sequence reads passing a quality filter were aligned to mouse genome (mm10) using Tophat.

Sample: HET_CD4_1

SAMN03892459 • SRS1008583 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Mice were euthanized and CD4 dendritic cells (CD11c+I-Ab+CD4+CD8-) were sorted from spleens on a FACSAriaIII (purity >99%). Equal number of DCs (5.0x105) in SRBC or PBS immunized group were then snap-frozen immediately for RNA isolation. Barcoded sequencing libraries were generated using 100ng of RNA with the Ovation RNA-seq System V2 and Encore Rapid Library System. RNA-seq for Illumina HiSeq 2500

Experiment attributes:

GEO Accession: GSM1828772

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 27.9M spots, 5.6G bases, 3Gb

Run	# of Spots	# of Bases	Size	Published
SRR2121685	27,928,438	5.6G	3Gb	2015-10-01

ID:: 1626428

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