Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted from the frozen human placentas using TRIZOL reagent following the manufacturer's protocol. The RNA yield and purity were determined using Agilent 2100 Bio-analyzer (Agilent Technologies, Santa Clara, CA, United States) and NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, United States). All RNA samples passed quality control. RNA libraries were prepared for sequencing using standard Illumina protocols