Instrument: Illumina MiSeq
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Cells and EVs were snap frozen and stored at -80 degrees Celsius until extraction. RNA was extracted from EVs and filament samples using standard methods for TRI-reagen LS (Sigma) and TRI-reagent (Sigma), respectively. Extracted RNA was treated with DNase I (ThermoFisher) and re-extracted with TRI-reagent LS. Coprecipitant GlycoBlueTM (ThermoFisher) was used for EV RNA samples for the first extraction and for all samples in the re-extraction. Libraries for sequencing were generated with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina together with NEBNext® Poly(A) mRNA Magnetic Isolation Module according to the manufacturer's instructions.