U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX11049138: GSM5352485: 2.4.1 d0827 exp_3; Rhodobacter sphaeroides 2.4.1; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 14.4M spots, 1.1G bases, 429Mb downloads

Submitted by: NCBI (GEO)
Study: Transcriptome analysis of total RNA from Rhodobacter sphaeroides 2.4.1 wildtype and StsR (RSs0827) deletion strain during different growth phases
show Abstracthide Abstract
The sRNA StsR (formerly RSs0827) was first described as an sRNA induced upon iron starvation and was later demonstrated to be the most highly induced RNA in late stationary phase in the phototrophic Alphaproteobacterium R. sphaeroides. StsR is under control of RpoHI/RpoHII and therefore induced by multiple stress factors. To investigate the function of this sRNA on the hole transcriptome of R. sphaeroides we performed RNAseq with cultures of the exponential growth (6 h) phase, stationary growth phase (72 h / 144 h) and outgrowth phase. Cultures were grown under microaerobic growth conditions in biological triplicates. Overall design: This data includes the transcriptome data of two different strains each in triplicates: Rhodobacter sphaeroides 2.4.1 (wildtype) and R. sphaeroides 2.4.1 StsR deletion strain (d0827). Total RNA of all strains was isolated and sequenced.
Sample: 2.4.1 d0827 exp_3
SAMN19513404 • SRS9117172 • All experiments • All runs
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was isolated using the hot phenol method and remaining DNA was removed by a DNaseI treatment (Invitrogen). RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM5352485
Links:
Runs: 1 run, 14.4M spots, 1.1G bases, 429Mb
Run# of Spots# of BasesSizePublished
SRR1471113914,395,0431.1G429Mb2021-08-06

ID:
14699376

Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...