GSM5282248: PE replicate 6 [RAW264.7]; Mus musculus; RNA-Seq - SRA

SRX10775272: GSM5282248: PE replicate 6 [RAW264.7]; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 36.3M spots, 2.8G bases, 1Gb downloads

Submitted by: NCBI (GEO)

Study: The lupus susceptibility allele DRB1*03:01 encodes a disease driving epitope [RAW264.7]

PRJNA727351 • SRP318481 • All experiments • All runs

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We report RNA sequencing data that shows the differential effects of HLA-DRb1*0301 (LE), HLA-DRb1*0401 (SE) and HLA-DRb1*0402 (PE) on macrophage gene expression under IFN? cell culture conditions. Overall design: mRNA profiles obtained from RAW264.7 cells cultured in presence of synthetic peptides coding the 65-79 region of HLA-DRb1*0301 (LE), HLA-DRb1*0401 (SE) or HLA-DRb*0402 (PE) and IFN?.

Sample: PE replicate 6 [RAW264.7]

SAMN19013964 • SRS8862462 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was isolated with Trizol and the Direct-ZolTM RNA miniprep from Zymo research. Genomic DNA was removed using the Turbo DNA-Free kit from Ambion. 200 ng total RNA was used to generate libraries with the Illumina TruSeq RNA Sample Prep Kit. RNA libraries were prepared for sequencing using standard Illumina protocols.

Experiment attributes:

GEO Accession: GSM5282248

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 36.3M spots, 2.8G bases, 1Gb

Run	# of Spots	# of Bases	Size	Published
SRR14424073	36,327,492	2.8G	1Gb	2022-08-04

ID:: 14314136

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