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SRX10775160: GSM5282282: SE replicate 2 [THP-1]; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 24.6M spots, 2.5G bases, 869.5Mb downloads

Submitted by: NCBI (GEO)
Study: The lupus susceptibility allele DRB1*03:01 encodes a disease driving epitope [THP-1]
PRJNA727352 • SRP318479 • All experiments • All runs
show Abstracthide Abstract
We report RNA sequencing data that shows the differential effects of HLA-DRb1*0301 (LE), HLA-DRb1*0401 (SE) and HLA-DRb1*0402 (PE) on macrophage gene expression under IFN? cell culture conditions. Overall design: mRNA profiles obtained from THP-1 cells cultured in presence of synthetic peptides coding the 65-79 region of HLA-DRb1*0301 (LE), HLA-DRb1*0401 (SE) or HLA-DRb*0402 (PE) and IFN?.
Sample: SE replicate 2 [THP-1]
SAMN19013673 • SRS8862090 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was isolated with RNeasy mini kit from Qiagen. Genomic DNA was removed using the Turbo DNA-Free kit from Ambion. 200 ng total RNA was used to generate libraries with the Illumina TruSeq RNA Sample Prep Kit. RNA libraries were prepared for sequencing using standard Illumina protocols.
Experiment attributes:
GEO Accession: GSM5282282
Links:
Runs: 1 run, 24.6M spots, 2.5G bases, 869.5Mb
Run# of Spots# of BasesSizePublished
SRR1442396124,571,0362.5G869.5Mb2022-08-04

ID:
14314024

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