GSM1715938: D8B rep1; Saccharomyces cerevisiae; RNA-Seq - SRA

SRX1066426: GSM1715938: D8B rep1; Saccharomyces cerevisiae; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 17.6M spots, 877.8M bases, 524.1Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Adaptive aneuploidy protects against thiol peroxidase deficiency by increasing respiration via key mitochondrial proteins

PRJNA287520 • SRP059681 • All experiments • All runs

show Abstracthide Abstract

We characterized the mechanisms that allow yeast cells to survive under conditions of thiol peroxidase deficiency. Two thiol peroxidase null yeast (delta8) strains independently acquired a second copy of chromosome XI and increased expression of genes encoded by it. Delta-8 strains were compared to the Wt-M11 strain, which is characterized by duplication of chr-XI. Overall design: Examination of thiol peroxidase-deficient yeast strains.

Sample: D8B rep1

SAMN03782980 • SRS966422 • All experiments • All runs

Organism: Saccharomyces cerevisiae

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Frozen aliquots were thawed and lysed in 400 μL of lysis buffer (mRNA DIRECT kit; Invitrogen). A 250-μL aliquot of magnetic beads and two rounds of purification were implemented according to the manufacturer’s protocol RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM1715938

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 17.6M spots, 877.8M bases, 524.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR2071258	17,555,733	877.8M	524.1Mb	2015-10-13

ID:: 1545725

SRA

Sequence Read Archive

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