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SRX10617525: GSM5244521: DIO DMSO-2; Mus musculus; RNA-Seq
4 ILLUMINA (NextSeq 500) runs: 49.9M spots, 3.8G bases, 1.4Gb downloads

Submitted by: NCBI (GEO)
Study: Next Generation Sequencing of DIO Liver steatosis DMSO vs NCT (HNF4a agonist)
PRJNA722504 • SRP315129 • All experiments • All runs
show Abstracthide Abstract
Mice rendered obese with high fat diet were treated by IP injection with N-trans caffeoyltyramine (NCT) (200 mg/kg bid) for 14 days, followed by harvesting of the liver for analysis, including RNA isolation. Overall design: High fat diet induced non-alcoholic fatty liver (NAFLD) treated with DMSO or NCT compound.
Sample: DIO DMSO-2
SAMN18761581 • SRS8715093 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Liver from DMSO or NCT treated mice was removed, flash frozen in liquid nitrogen, and RNA harvested using Trizol reagent. PolyA RNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina®(NEB, Ipswich MA). Libraries were pooled and single end sequenced (1X75) on the Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA).
Experiment attributes:
GEO Accession: GSM5244521
Links:
Runs: 4 runs, 49.9M spots, 3.8G bases, 1.4Gb
Run# of Spots# of BasesSizePublished
SRR1425507512,286,800927.9M343.5Mb2021-04-17
SRR1425507612,242,270924.6M341.8Mb2021-04-17
SRR1425507712,692,074958.5M353.2Mb2021-04-17
SRR1425507812,704,383959.5M354.3Mb2021-04-17

ID:
14094899

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