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SRX10615783: GSM5244259: Lats-Plp Tumor 2 Grade II; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 93.6M spots, 18.6G bases, 2.3Gb downloads

Submitted by: NCBI (GEO)
Study: A genetic mouse model of malignant peripheral nerve sheath tumor with postnatal Nf1 and p53 loss recapitulates the histology and transcriptome of human tumors
show Abstracthide Abstract
We established a new genetically engineered mouse (GEM) model of malignant peripheral nerve sheath tumors (MPNST) based on postnatal deletion of a Nf1;Trp53 cis-conditional allele by the tamoxifen-inducible Plp-CreER (NP-Plp). We also generated two Lats1;2 conditional knockout models by using Nestin-Cre (Lats-Nes) and Plp-CreER (Lats-Plp), both of which also develop tumors similar to MPNST (GEM-PNST). To evaluate these models, transcriptome analyses were performed to compare these models and with human MPNST, plexiform neurofibromas (PNF), and neurofibromas (NF). Overall design: Transcriptome comparison of various mouse models of maligant peripheral nerve sheath tumor (NP-Plp, NPcis, Lats-Nes, and Lats-Plp) and human MPNST, PNF, and NF tumors.
Sample: Lats-Plp Tumor 2 Grade II
SAMN18757508 • SRS8713377 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was isolated using the Direct-zol RNA Miniprep kit (Zymo), quantified by Qubit 3.0 Fluorometer, and quality checked by 4200 TapeStation High Sensitivity RNA ScreenTape assay (Agilent). Libraries were prepared from 350 ng of total RNA using the TruSeq Stranded Total RNA Library Prep Kit (Illumina 20020599) Libraries were sequenced on Illumina NovaSeq 6000, obtaining 100 million 100-bp paired-end reads.
Experiment attributes:
GEO Accession: GSM5244259
Links:
Runs: 1 run, 93.6M spots, 18.6G bases, 2.3Gb
Run# of Spots# of BasesSizePublished
SRR1425332493,604,51818.6G2.3Gb2024-03-25

ID:
14093157

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