show Abstracthide AbstractWe analyzed the transcriptomes of different primary microglia cultures from adult rhesus macaques, and compared them with those of ex vivo microglia Overall design: Primary microglia cultured with M-CSF, IL-34, M-CSF + TGFb, IL-34 + TGFb (n=4). Primary microglia cultured with serum + M-CSF, serum (4days) + different serum-free wash out regimes (4, 11 and 18 days), and serum-free medium (22 days) (n=4). Primary microglia cultured with serum + M-CSF, with serum for 4 days and subsequently cultured in serum-free medium for 11 days, and microglia cultured in spheres together with oligodendrocytes and radial glia (n=4). Freshly isolated microglia (ex vivo) (n=4)