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SRX10484302: GSM5220431: control_0_Rep2_Lib1; Drosophila melanogaster; RNA-Seq
3 ILLUMINA (NextSeq 500) runs: 15M spots, 1.1G bases, 438.1Mb downloads

Submitted by: NCBI (GEO)
Study: Timecourse RNAseq for Drosophila TBI
show Abstracthide Abstract
Comparison of post-injury (sham versus severe TBI) gene expression changes using a Drosophila head-specific TBI model. Overall design: 64 total libraries were prepared from 52 unique RNA samples, representing the molecular response to traumatic brain injury spanning 9 timepoints from 0 through 15 days post-injury. Samples consist of heads isolated from 3d old uninjured males (controls, 0 post injury timepoint) and paired sham vs TBI injured animals (3d old males) at 1h, 3h, 6h, 12h, 24h, 3d, 7d, 10d, and 15d post-injury. All times/conditions have 3 biological replicates, with the exception of 15d due to library prep failure. Library preparation took place on two separate days (noted as library 1 or library 2), hence libraries for control and sham/TBI 24 h timepoints were prepared twice and used for batch effect correct.
Sample: control_0_Rep2_Lib1
SAMN18542769 • SRS8611738 • All experiments • All runs
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: whole flies were flash frozen in liquod nitrogen; heads (n=20/sample) were dissociated by vortexing then collected. Total RNA was extracted by TRIzol/chloroform. sample libraries were prepared in two batches (separate days) using the same Illumina TruSeq Stranderd Sample Kit (Cat# RS-122-2103) RNA-seq; sequenced on Illumina NextSeq using NextSeq® 500/550 High Output Kit v2 (75 cycles; FC-404-2005)
Experiment attributes:
GEO Accession: GSM5220431
Links:
Runs: 3 runs, 15M spots, 1.1G bases, 438.1Mb
Run# of Spots# of BasesSizePublished
SRR141136264,853,921357.2M140.7Mb2021-07-22
SRR141136275,150,598379.1M150.5Mb2021-07-22
SRR141136285,010,984368.9M146.9Mb2021-07-22

ID:
13898461

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