Instrument: Illumina HiSeq 2500
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: other
Layout: PAIRED
Construction protocol: Total RNA of RNA-seq samples were homogenized in 1 ml TRIzol (Accurate Biotechnology) and precipitated by isopropyl alcohol. Total DNA of 16S-seq samples were extracted from Drosophila midgut using TIANamp genomic DNA kit (Tiangen, DP304) following the manufacturer's protocol. After total RNAs of each sample were extracted, the mRNAs enriched by Oligo(dT) beads were fragmented using fragmentation buffer and reverse transcribed into cDNAs with random primers. Second-strand cDNA were synthesized by DNA polymerase I, RNase H, dNTP, and buffer. The cDNA fragments were purified with QiaQuick PCR extraction kit (Qiagen), and were end repaired, poly(A) added, and ligated to Illumina sequencing adapters. The ligation products were size selected by agarose gel electrophoresis, PCR amplified, and sequenced using Illumina HiSeq2500 by Genedenovo Biotechnology Co, Ltd.