GSM5173704: N. crassa 240min [P3-2]; Neurospora crassa; RNA-Seq - SRA

SRX10354702: GSM5173704: N. crassa 240min [P3-2]; Neurospora crassa; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 29.6M spots, 6G bases, 1.7Gb downloads

Submitted by: NCBI (GEO)

Study: Gene expression during conidia germination in Neurospora crassa on PDA

PRJNA714875 • SRP310858 • All experiments • All runs

show Abstracthide Abstract

Conidia germination is critical for fungi to colonize various habitats. We sampled RNA expression at four stages of conidia germination, including fresh conidia (15min), polar growth (120min), doubling of long axis (240min), and first hyphal branching (360min) in Neurospora crassa. Cultures were made onPDA, iand two biological replicates were collected for all data points. The growth was under a labratory condition of 25C and constant light. Overall design: mRNA were sampled and compared from four time points across conida germination in Neurpsora crassa on different media

Sample: N. crassa 240min [P3-2]

SAMN18319044 • SRS8472982 • All experiments • All runs

Organism: Neurospora crassa

Library:

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: For transcriptomic analysis, all tissues and perithecia were immediately and rapidly frozen in liquid nitrogen as they were sampled, then stored at -80°C. RNA was isolated from homogenized mycelia with TRI REAGENT (Invitrogen) and mRNA was purified using Dynabeads oligo(dT) magnetic separation (Invitrogen) Standard Illumina protocal

Experiment attributes:

GEO Accession: GSM5173704

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 29.6M spots, 6G bases, 1.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR13977093	29,603,591	6G	1.7Gb	2022-03-19

ID:: 13645486

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