GSM5066172: HUDEP-2_IgG; Homo sapiens; OTHER - SRA

SRX10031372: GSM5066172: HUDEP-2_IgG; Homo sapiens; OTHER
1 ILLUMINA (NextSeq 500) run: 5.8M spots, 475.7M bases, 199.1Mb downloads

Submitted by: NCBI (GEO)

Study: Genome-wide ZNF410 binding sites identified by CUT&RUN in HUDEP-2, human CD34+ HSPCs and MEL cells.

PRJNA699604 • SRP304785 • All experiments • All runs

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We examined the chromatin occupancy of ZNF410 by conducting CUT&RUN. We used an HA antibody to probe for epitope tagged ZNF410 in HUDEP-2 cells. We used a ZNF410 antibody to probe for endogenous ZNF410 in HUDEP-2 cells, human CD34+ hematopoietic stem and progenitor cell (HSPC) derived erythroid precursors and mouse erythroleukemia (MEL) cells. Overall design: Genomic enrichment for ZNF410 binding was determined using IgG as the negative control.

Sample: HUDEP-2_IgG

SAMN17800080 • SRS8199048 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: OTHER

Source: GENOMIC

Selection: other

Layout: PAIRED

Construction protocol: Genomic DNA was isolated and prepared following the CUT&RUN protocol from Skene PJ, et al. Nat Protoc. 2018, PMID: 29651053. Libraries were prepared using the NEBNext Ultra™ II DNA Library Prep Kit for Illumina following the protocol from Liu N, et al. Cell 2018, PMID: 29606353. CUT&RUN, Paired-end 42bp sequencing.

Experiment attributes:

GEO Accession: GSM5066172

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 5.8M spots, 475.7M bases, 199.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR13634070	5,800,883	475.7M	199.1Mb	2021-02-06

ID:: 13158786

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