GSM5062124: Control_B; Perkinsus marinus; RNA-Seq - SRA

SRX10016435: GSM5062124: Control_B; Perkinsus marinus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 65.2M spots, 19.7G bases, 6.5Gb downloads

Submitted by: NCBI (GEO)

Study: A dual omics approach to evaluate transcriptional and metabolic responses during lipid deprivation in an oyster parasite Perkinsus marinus

PRJNA699315 • SRP304612 • All experiments • All runs

show Abstracthide Abstract

In this study, we explored the metabolic processes of P. marinus under lipid deprived conditions to elucidate the interchanging flux of lipid and carbohydrate metabolism. Though P. marinus can synthesize their own lipids from available nutrients, they display a slower growth in media not supplemented with lipids as opposed to media with lipids. Under these conditions, using transcriptomics, we surprisingly observed evidence of stimulated lipid degradation through increased transcription of two core ß-oxidation pathway enzymes. Overall design: We sought to examine the transciptional effects of lipid withdrawal from growth media using RNA seq methodologies.

Sample: Control_B

SAMN17773443 • SRS8184743 • All experiments • All runs

Organism: Perkinsus marinus

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: P. marinus cells were harvested from day 11 lipid replete and lipid deplete media for each biological replicate. Cells were pelleted at 3,900 RPM for 10 minutes at 4 C° using an Eppendorf 5810R Centrifuge. P. marinus total RNA was extracted per manufacturer's protocol using the Qiagen RNeasy mini- kit; RNA quality was verified on a 1% percent agarose gel and visualized using a UV transilluminator. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM5062124

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 65.2M spots, 19.7G bases, 6.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR13623350	65,227,044	19.7G	6.5Gb	2021-02-05

ID:: 13143849

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