ChIP-Seq analysis of H3K27ac in human Mobilized CD34 cells - SRA

SRX088800: ChIP-Seq analysis of H3K27ac in human Mobilized CD34 cells
3 ILLUMINA (Illumina HiSeq 2000) runs: 30.5M spots, 1.1G bases, 794.8Mb downloads

Design: Single read - Illumina

Submitted by: Gene Expression Omnibus (GEO)

Study: BI Human Reference Epigenome Mapping Project

• SRP000996 • All experiments • All runs

show Abstracthide Abstract

The NIH Roadmap Epigenomics Mapping Consortium aims to produce a public resource of epigenomic maps for stem cells and primary ex vivo tissues selected to represent the normal counterparts of tissues and organ systems frequently involved in human disease. Characterization of the reference epigenome in humans by use of ChIP-Seq in a diverse panel of ES cells, tissue stem cells, reprogrammed stem cells, primary cells and tissues.

Sample: Generic sample from Homo sapiens

SAMN00113437 • SRS114970 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: DNA_Lib 551

Instrument: Illumina HiSeq 2000

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Spot descriptor:

1 forward

Experiment attributes: (show all 14 attributes...) (hide...)

CHIP_PROTOCOL_BEAD_TYPE: Protein A-Sepharose 4B

CHIP_ANTIBODY: H3K27ac

CHIP_PROTOCOL_CHROMATIN_AMOUNT: 0.5 to 3 million cells

EXTRACTION_PROTOCOL: cell/nuclei lysis

CHIP_PROTOCOL_ANTIBODY_AMOUNT: ~1 ug

CHIP_PROTOCOL_BEAD_AMOUNT: 10 ul bed volume

CHIP_PROTOCOL: Bernstein_BROAD_ENCODE_protocol

EXTRACTION_PROTOCOL_SONICATION_CYCLES: 1

CHIP_ANTIBODY_CATALOG: ab4729

CHIP_ANTIBODY_PROVIDER: Abcam

CHIP_ANTIBODY_LOT: 509313

EXPERIMENT_TYPE: Histone H3K27ac

EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Branson

GEO Accession: GSM772885

Pipeline: show...hide...

Name	Step	Prev Step	Program	Version
BASE_CALLS	1	NIL	GAPipeline	RTA1.10.36
QUALITY_SCORES	2	1	GAPipeline	RTA1.10.36

Links:

External link: EDACC Genboree Page

External link: GEO Web Link

Runs: 3 runs, 30.5M spots, 1.1G bases, 794.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR1067168	9,167,320	330M	239Mb	2014-01-02
SRR1067231	11,317,203	407.4M	294.4Mb	2014-01-02
SRR1067299	10,025,834	360.9M	261.4Mb	2014-01-02

ID:: 101016

SRA

Sequence Read Archive

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