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SRX088697: ChIP-Seq analysis of H3K4me3 in human CD15 cells
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 40M spots, 1.4G bases, 1.1Gb downloads

Design: Single read - Illumina
Submitted by: Gene Expression Omnibus (GEO)
Study: BI Human Reference Epigenome Mapping Project
 • SRP000996 • All experiments • All runs
show Abstracthide Abstract
The NIH Roadmap Epigenomics Mapping Consortium aims to produce a public resource of epigenomic maps for stem cells and primary ex vivo tissues selected to represent the normal counterparts of tissues and organ systems frequently involved in human disease. Characterization of the reference epigenome in humans by use of ChIP-Seq in a diverse panel of ES cells, tissue stem cells, reprogrammed stem cells, primary cells and tissues.
Sample: Generic sample from Homo sapiens
SAMN00012132 • SRS065974 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: Lib EpiLabSPL Row 1797
Instrument: Illumina Genome Analyzer IIx
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Spot descriptor:
1  forward

Experiment attributes: (show all 14 attributes...) (hide...)
CHIP_PROTOCOL_BEAD_TYPE: Protein A-Sepharose 4B
CHIP_ANTIBODY: H3K4me3
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 10 million cells
EXTRACTION_PROTOCOL: SDS lysis
CHIP_PROTOCOL_ANTIBODY_AMOUNT: 2-15 ug
CHIP_PROTOCOL_BEAD_AMOUNT: 70 ul bed volume
CHIP_PROTOCOL: Bernstein_BROAD_ENCODE_protocol
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 1
CHIP_ANTIBODY_CATALOG: 07-473
CHIP_ANTIBODY_PROVIDER: Millipore
CHIP_ANTIBODY_LOT: DAM1623866
EXPERIMENT_TYPE: Histone H3K4me3
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Bioruptor
GEO Accession: GSM772782
Pipeline: show...hide...
NameStepPrev StepProgramVersion
BASE_CALLS1NILGAPipeline1.8.0
QUALITY_SCORES21GAPipeline1.8.0
Links:
External link:
External link:
Runs: 1 run, 40M spots, 1.4G bases, 1.1Gb
Run# of Spots# of BasesSizePublished
SRR106710640,022,4211.4G1.1Gb2014-01-02

ID:
100913

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