ChIP-Seq analysis of H3K27me3 in human fetal heart cells - SRA

SRX031444: ChIP-Seq analysis of H3K27me3 in human fetal heart cells
1 ILLUMINA (Illumina Genome Analyzer II) run: 21.4M spots, 770.2M bases, 604.7Mb downloads

Design: Single read - Illumina

Submitted by: Gene Expression Omnibus (GEO)

Study: BI Human Reference Epigenome Mapping Project: ChIP-Seq in human subject

• SRP001534 • All experiments • All runs

show Abstracthide Abstract

Characterization of the reference epigenome in humans by use of ChIP-Seq in a diverse panel of ES cells, tissue stem cells, reprogrammed stem cells, primary cells and tissues.

Sample: Generic sample from Homo sapiens

SAMN00113446 • SRS114979 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: Lib:XZ:20100129:20--ChIP:XZ:20100125:20:fetal heart:H3K27me3

Instrument: Illumina Genome Analyzer II

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Spot descriptor:

1 forward

Experiment attributes: (show all 14 attributes...) (hide...)

CHIP_PROTOCOL_BEAD_TYPE: Protein A-Sepharose 4B

CHIP_ANTIBODY: H3K27me3

CHIP_PROTOCOL_CHROMATIN_AMOUNT: 10 million cells

EXTRACTION_PROTOCOL: SDS lysis

CHIP_PROTOCOL_ANTIBODY_AMOUNT: 2-15 ug

CHIP_PROTOCOL_BEAD_AMOUNT: 70 ul bed volume

CHIP_PROTOCOL: Bernstein_BROAD_ENCODE_protocol

EXTRACTION_PROTOCOL_SONICATION_CYCLES: 1

CHIP_ANTIBODY_CATALOG: 07-449

CHIP_ANTIBODY_PROVIDER: Millipore

CHIP_ANTIBODY_LOT: DAM1514011

EXPERIMENT_TYPE: Histone H3K27me3

EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Bioruptor

GEO Accession: GSM621450

Links:

External link: EDACC Genboree Page

External link: GEO Web Link

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 21.4M spots, 770.2M bases, 604.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR1068384	21,394,678	770.2M	604.7Mb	2014-01-03

ID:: 38347

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity