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SRX026695: GSM587250: Normal tail tissue, specimen "G1_2-2"
1 ILLUMINA (Illumina Genome Analyzer II) run: 5.1M spots, 208.4M bases, 126.5Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Highly Aneuploid Zebrafish Malignant Peripheral Nerve Sheath Tumors have Genetic Alterations Similar to Human Cancers
show Abstracthide Abstract
Aneuploidy is a hallmark of human cancers, but most mouse cancer models lack the extensive aneuploidy seen in many human tumors. The zebrafish is becoming an increasingly popular model for studying cancer. Here we report that malignant peripheral nerve sheath tumors (MPNSTs) that arise in zebrafish as a result of mutations in either ribosomal protein (rp) genes or in p53 are highly aneuploid. Karyotyping reveals that these tumors frequently harbor near-triploid numbers of chromosomes, and they vary in chromosome number from cell-to-cell within a single tumor. Using array CGH (aCGH) we found that, as in human cancers, certain fish chromosomes are preferentially over-represented while others are under-represented in many MPNSTs. In addition, we detected evidence for recurrent sub-chromosomal amplifications and deletions that may contain genes involved in cancer initiation or progression. One striking amplification included the c-met proto-oncogene. Our results suggest that the zebrafish may be a valuable model in which to study aneuploidy in human cancer and in which to identify candidate genes that may act as drivers in fish and potentially also in human tumors. For GSM587250-GSM587255: This Illumina sequencing data is for validation of a larger array-CGH dataset (GSM580580-GSM580621). Overall design: For GSM580580-GSM580621: Two-condition (x3) experiment: (1) genomic DNA: MPNST (rp gene mutation) vs normal tail : 31 replicates; (2) MPNST (p53 mutation) vs normal tail: 5 replicates; (3) normal tail vs. normal tail (6 replicates) For GSM587250-GSM587255: Six samples total, in three pairs of tumor (MPNST) and normal tail control
Sample: Normal tail tissue, specimen "G1_2-2"
SAMN00113238 • SRS114771 • All experiments • All runs
Organism: Danio rerio
Library:
Name: GSM587250: Normal tail tissue, specimen "G1_2-2"
Instrument: Illumina Genome Analyzer II
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: SINGLE
Spot descriptor:
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Experiment attributes:
GEO Accession: GSM587250
Links:
External link:
Runs: 1 run, 5.1M spots, 208.4M bases, 126.5Mb
Run# of Spots# of BasesSizePublished
SRR0655345,081,964208.4M126.5Mb2010-09-15

ID:
32556

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