WGS project for strain SA-279 Ancalomicrobiaceae bacterium - SRA

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SRX5413170: WGS project for strain SA-279 Ancalomicrobiaceae bacterium
1 ILLUMINA (Illumina MiSeq) run: 862,597 spots, 431.3M bases, 246.7Mb downloads

Design: For the whole genome project of strain SA-279, the genomic DNA was extracted with the DNeasy PowerLyzer Microbial Kit (Qiagen) including an RNase A treatment at 37 C for 20 min. Illumina sequencing was performed by the Genomics Facility RTSF, Michigan State University (USA) with the following main steps: library preparation using the SMARTer ThruPLEX DNA-Seq kit (Takara); quality control using a combination of Qubit dsDNA HS assay (Thermo Fisher Scientific), 4200 TapeStation High Sensitivity DNA 1000 assay (Agilent) and the Illumina Library Quantification qPCR kit (Kapa Biosystems); sequencing which was performed in a 2x250 bp paired-end format using a MiSeq Standard v2 flow cell and a MiSeq 500 cycle v2 reagent cartridge (Illumina).

Submitted by: Eotvos Lorand University

Study: Siculibacillus lacustris strain:SA-279 Genome sequencing and assembly

PRJNA523705 • SRP186666 • All experiments • All runs

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Ancalomicrobiaceae bacterium strain SA-279 genome sequencing and assembly

Sample: Microbe sample from Ancalomicrobiaceae

SAMN10986326 • SRS4394561 • All experiments • All runs

Organism: Siculibacillus lacustris

Library:

Name: SA-279

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 862,597 spots, 431.3M bases, 246.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR8614134	862,597	431.3M	246.7Mb	2019-02-23

ID:: 7320870

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