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SRX11050033: feces sample
1 PACBIO_SMRT (PacBio RS II) run: 37,089 spots, 55.6M bases, 13.6Mb downloads

Design: 16s full-length sequencing
Submitted by: Beijing Institute of Microbiology and Epidemiology
Study: B7 family molecule VSIG4 regulates intestinal anti-Enterohemorrhagic Escherichia coli immunity by altering gut flora diversity
show Abstracthide Abstract
As an essential member of the B7 family, VSIG4 (V-set and immunoglobulin domain-containing 4) is expressed explicitly in tissue-resident macrophages (TRM) of digestive organs and plays an essential role in maintaining the homeostasis of the environmental immune system. Here we demonstrate that gene-targeted VSIG4-deficient mice infected with EHEC (Enterohemorrhagic Escherichia coli) display reduced bacterial burden. To address the functional protection of VSIG4 against EHEC infection. We collected mouse feces and used the High-throughput 16S rRNA gene amplicon analysis to detect. A total of 657330 sequences were sequenced on the PacBio platform, with an average length of 1498bp. We found that VSIG4-deficiency could alter the gut microbiota by increasing diversity and shifting community composition, which G_Akkermansia and G_Oscillospiraceae increased significantly. These findings extend a prior observation that VSIG4-deficiency reduced bacterial burdon by changing the gut microbiota diversity or shifting community composition.
Sample: feces
SAMN19513676 • SRS9118065 • All experiments • All runs
Library:
Name: Het8
Instrument: PacBio RS II
Strategy: AMPLICON
Source: METAGENOMIC
Selection: PCR
Layout: SINGLE
Runs: 1 run, 37,089 spots, 55.6M bases, 13.6Mb
Run# of Spots# of BasesSizePublished
SRR1471203537,08955.6M13.6Mb2021-06-15

ID:
14700380

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