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SRX4607551: GSM3356752: m6A INPUT Rep1 20H; Homo sapiens; RIP-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 46.6M spots, 11G bases, 4.9Gb downloads

Submitted by: NCBI (GEO)
Study: m6A-RNA mapping, SND1-RNA binding profile mapping and SND1-depletion in KSHV-infected B-lymphocytes
show Abstracthide Abstract
We have mapped m6A sites and endogenous SND1 binding sites in the viral and cellular transcriptome using TREx BCBL1-Rta cells. In addition, we have depleted SND1 in TREx BCBL1-Rta cells and BCBL1 cells and analyzed their RNA expression profile both during latency and lytic replication. Overall design: Identification of m6A sites in TREx BCBL1-Rta cells at 0h, 8 and 20 h post-lytic reactivation. SND1 transcriptome-wide binding profile in TREx BCBL1-Rta cells at 0h, 8 and 20 h post-lytic reactivation. RNA expression profile in scramble and SND1-depleted cells in both latent and reactivated TREx BCBL1-Rta cells for 24 h. Same RNA expression profile in shRNA scramble and shRNA SND1-depleted BCBL1 cells.
Sample: m6A INPUT Rep1 20H
SAMN09910079 • SRS3711955 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2500
Strategy: RIP-Seq
Source: TRANSCRIPTOMIC
Selection: other
Layout: PAIRED
Construction protocol: Total RNA was used for m6A-seq and RNA-seq. For SND1-seq, input libraries were made from total RNA isolated with TRIzol (Thermo Fisher) and RIP libraries were made from sonicated SND1 enriched RNA which had been purified with TRIzol LS (Thermo Scientific). m6A-seq libraries were made using NEBNext Ultra kit (NEB) according to the manufacturer's protocol. RIP-seq and RNA-seq libraries were made using TruSeq Stranded Total RNA library production kit (Illumina)
Experiment attributes:
GEO Accession: GSM3356752
Links:
Runs: 1 run, 46.6M spots, 11G bases, 4.9Gb
Run# of Spots# of BasesSizePublished
SRR775163046,625,03911G4.9Gb2019-10-16

ID:
6227577

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