assay type: RNA-seq of coding RNA
sample storage: frozen, -70 freezer
sample storage processing: cryopreservation in liquid nitrogen (dead tissue)
sampling to preparation interval: 49 months
experimental protocol:
https://support.illumina.com/content/dam/illumina- (show full text...)(hide...)https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
extraction protocol:
https://www.qiagen.com/se/resources/resourcedetail (show full text...)(hide...)https://www.qiagen.com/se/resources/resourcedetail?id=14e7cf6e-521a-4cf7-8cbc-bf9f6fa33e24
library preparation location:
Centro de Genomica Funcional ESALQ-USP Piracicaba (show full text...)(hide...)Centro de Genomica Funcional ESALQ-USP Piracicaba Brazil
library preparation location longitude: -22.71 decimal degrees
library preparation location latitude: -47.63 decimal degrees
library preparation date: 2017-10-18 YYYY-MM-DD
sequencing location:
Functional Genomic applied to Agriculture ESALQ-US (show full text...)(hide...)Functional Genomic applied to Agriculture ESALQ-USP Piracicaba Brazil
sequencing location longitude: -22.71 decimal degrees
sequencing location latitude: -47.63 decimal degrees
sequencing date: 2017-10-25 YYYY-MM-DD
experiment target: polyA RNA
rna preparation 3' adapter ligation protocol:
https://support.illumina.com/content/dam/illumina- (show full text...)(hide...)https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
rna preparation 5' adapter ligation protocol:
https://support.illumina.com/content/dam/illumina- (show full text...)(hide...)https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
library generation pcr product isolation protocol:
https://support.illumina.com/content/dam/illumina- (show full text...)(hide...)https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
preparation reverse transcription protocol:
https://support.illumina.com/content/dam/illumina- (show full text...)(hide...)https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
library generation protocol:
https://support.illumina.com/content/dam/illumina- (show full text...)(hide...)https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
read strand: mate 1 sense
rna purity - 260:280 ratio: 2.14
rna purity - 260:230 ratio: not collected
rna integrity number: 9.2