Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: total RNAs from dormant, reactivating, and active cambium meristem were isolated using the Trizol reagent The library was constructed using Small RNA Sample Pre Kit. Based on the 3 'and 5' special structures of Small RNA (complete phosphate groups on the 5 'end and hydroxyl groups on the 3' end), total RNA was used as a starting sample and Small Both ends of the RNA were spliced and cDNA was reverse transcribed. Followed by PCR amplification, PAGE gel electrophoresis separation of the target DNA fragment, gel recovery obtained is the cDNA library