RAD-seq of Pungitius hellenicus: female - SRA

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SRX3384047: RAD-seq of Pungitius hellenicus: female
1 ILLUMINA (Illumina HiSeq 2000) run: 3.7M spots, 727.1M bases, 522.9Mb downloads

Design: Genomic DNA was extracted from ethanol preserved fin clips or muscle tissue using the standard phenol-chloroform method. Approximately 100 ng of genomic DNA was isolated for RAD library construction following the protocol of Baird et al. (2008). DNA was fragmented with restriction enzyme (PstI). P1 adapter with forward amplification and sequencing primers and sample specific barcode were ligated to the digested DNA fragments. Barcoded samples were sheared randomly and the P2 adapter was added. DNA fragments of 300 to 500 bp in length were gel purified after selective PCR enrichment step. Barcoded RAD samples were then pooled and sequenced using the Illumina HiSeq2000 platform with 100 bp paired-end strategy.

Submitted by: Institute of Zoology, Chinese Academy of Science

Study: Pungitius RAD-seq sequencing

PRJNA418047 • SRP124811 • All experiments • All runs

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The global Pungitius stickleback evolutionary study.

Sample:

SAMN08013781 • SRS2679417 • All experiments • All runs

Organism: Pungitius hellenicus

Library:

Name: G786

Instrument: Illumina HiSeq 2000

Strategy: RAD-Seq

Source: GENOMIC

Selection: Restriction Digest

Layout: PAIRED

Runs: 1 run, 3.7M spots, 727.1M bases, 522.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR6281980	3,709,830	727.1M	522.9Mb	2020-12-31

ID:: 4721751

SRA

Sequence Read Archive

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