RNA-Seq of Diporeia Hoyi: Diporeia Hoyi Sample #359 - SRA

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SRX2638461: RNA-Seq of Diporeia Hoyi: Diporeia Hoyi Sample #359
1 ILLUMINA (Illumina MiSeq) run: 1.5M spots, 731.7M bases, 454.3Mb downloads

Design: Nucleic acids were extracted from individual amphipods via ZR-Duetª DNA/RNA MiniPrep kit. RNA fractions were further enzymatically digested with TurboDNase for 15min to reduce co-extracted DNA. Ribosomal RNA was depleted via mRNA-ONLYª mRNA Isolation Kit, and remaining RNA was reverse transcribed and amplified via TransPlex¨ Complete Whole Transcriptome Amplification Kit. Resulting cDNA libraries were quantified via Pico Green fluorescence and prepared for sequencing using a Nextera XT DNA Library Preparation Kit. Resulting libraries were subjected to 2x250bp paired-end sequencing on an Illumina Miseq.

Submitted by: Cornell University

Study: Diporeia Hoyi Transcriptome

PRJNA379017 • SRP101850 • All experiments • All runs

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This study is an initial investigation of the relationship between benthic meiofauna and circular rep-encoding ssDNA (CRESS-DNA) viruses in the Laurentian Great Lakes. Goals of whole-amphipod transcriptome sequencing include: (1) elucidate the relationship between presence/load of CRESS-DNA virus LM29173 and Diporeia gene expression (2) determine if gene expression profiles vary by amphipod population, and (3) identify innate-immunity related genes putatively associated with LM29173 presence.

Sample: Sample #359

SAMN06565227 • SRS2046452 • All experiments • All runs

Organism: Diporeia hoyi

Library:

Name: D359

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 1.5M spots, 731.7M bases, 454.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR5341786	1,457,582	731.7M	454.3Mb	2017-06-24

ID:: 3816533

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