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SRX1814975: GSM2183950: 3.CCIC-shG9a-DNG9a; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 90.3M spots, 4.4G bases, 1.7Gb downloads

Submitted by: NCBI (GEO)
Study: HiSeq Chip-seq analysis of whole genomic landscape of G9a and H3K9me2-targeted in CCICs
PRJNA324111 • SRP075976 • All experiments • All runs
show Abstracthide Abstract
Purpose: to demonstrate that G9a maintain CCICs functional triats is independent of its methyltransferase activity. Method: ChIP-seq was applied to CCICs to elucidate the nature of G9a and H3K9me2-regulated differential gene targets by Illumina/Soleza Genome analyzer Results: It was shown that ghe 4,902 G9a-and H3K9me2-targeted genes are broadly idstributed throughout the genome, and both are co-localized at most of the sites in CCICs. Overall design: G9a and H3K9me2-targeted in CCICs
Sample: 3.CCIC-shG9a-DNG9a
SAMN05195187 • SRS1477881 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 4000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Millipore EZ-ChIP #17-371 kit (1)DNA-end repair,3'-dA overhang and ligation of methylated sequencing adaptor.(2)PCR amplification and size selection (usually 100-300bp,including adaptorsequence).(3)Qualified library forsequencing. Which follow illumina instruction
Experiment attributes:
GEO Accession: GSM2183950
Links:
Runs: 1 run, 90.3M spots, 4.4G bases, 1.7Gb
Run# of Spots# of BasesSizePublished
SRR361875390,260,0804.4G1.7Gb2016-08-10

ID:
2593611

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