mulitplexed mSTARR-seq of Homo sapiens - SRA

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SRX25367524: mulitplexed mSTARR-seq of Homo sapiens
1 ILLUMINA (Illumina NovaSeq 6000) run: 4.3M spots, 866.4M bases, 273.3Mb downloads

Design: Msp1 digested human genomic DNA assembled into the mSTARR plasmid, experimentally methylated, and assessed for regulatory activity via abundance of transcribed RNA relative to input DNA. Sequencing libraries extracted from plasmid DNA and RNA prepared using targetted PCR

Submitted by: Vanderbilt University

Study: Multiplexed mSTARR-seq identifies methylation-dependent genetics effects on regulatory element function

PRJNA1137064 • SRP520627 • All experiments • All runs

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The goal was to input naturally occurring genetic variation (from 1000 Genomes individuals) into the mSTARR-seq MPRA to test for methylation-dependent genetic effects. Half of replicates were experimentally methylated and half were left unmethylated so that regulatory activity of input fragments (abundance of RNA relative to amount of input DNA) could be assessed in both contexts.

Sample: DNA/RNA extracted from multiplexed mSTARR-seq assay

SAMN42566169 • SRS22033264 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: L51981

Instrument: Illumina NovaSeq 6000

Strategy: OTHER

Source: GENOMIC

Selection: Restriction Digest

Layout: PAIRED

Runs: 1 run, 4.3M spots, 866.4M bases, 273.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR29870503	4,289,287	866.4M	273.3Mb	2024-08-20

ID:: 33753251

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