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SRX24850361: Gut microbiota sequence of juvenile yellowfin seabream
1 ILLUMINA (Illumina HiSeq 2500) run: 131,482 spots, 63.6M bases, 19.4Mb downloads

Design: DNA was extracted from each pooled sample using Qiagens QIAamp DNA Kits according to instructions. The concentration of DNA was measured with a NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific, USA). The 16S rDNA V3 and V4 variable regions of the ribosomal RNA gene were amplified by using barcoded fusion primers (341F 5-CCTAYGGGRBGCASCAG-3 and 806R 5-GGACTACNNGGGTATCTAAT-3). PCR reactions were conducted in triplicate for each sample as follows: initial denaturation at 95 for 5 minutes, followed by 30 cycles of denaturation at 95 for 1 minute, annealing at 60 for 1 minute, and elongation at 72 for 1 minute. The final elongation was carried out at 72 for 7 minutes. PCR products were extracted from 2% agarose gels, purified using the QIAquick Gel Extraction Kit from Qiagen, and quantified using the ABI StepOnePlus Real-Time PCR System from Life Technologies, USA. Finally, the clean-up DNA was pooled in equimolar and paired-end (PE250) sequenced using an Illumina HiSeq 2500 sequencing platform (Illumina, USA) at Gene Denovo Biotechnology Co. (Guangzhou, China) following standard protocols.
Submitted by: Guangdong Academy of Sciences
Study: Gut microbiota seq for yellowfin seabream
PRJNA1121642 • SRP512728 • All experiments • All runs
show Abstracthide Abstract
Gut microbiota seq for yellowfin seabream under different salinities.
Sample:
SAMN41763293 • SRS21561292 • All experiments • All runs
Organism: Acanthopagrus latus
Library:
Name: FI_BW1
Instrument: Illumina HiSeq 2500
Strategy: AMPLICON
Source: OTHER
Selection: RANDOM PCR
Layout: PAIRED
Runs: 1 run, 131,482 spots, 63.6M bases, 19.4Mb
Run# of Spots# of BasesSizePublished
SRR29334220131,48263.6M19.4Mb2024-06-08

ID:
33184701

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