Lactobacillus crispatus UMB4277 - SRA

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SRX24349005: Lactobacillus crispatus UMB4277
1 ILLUMINA (NextSeq 2000) run: 1.9M spots, 564.3M bases, 199.2Mb downloads

Design: DNA was extracted from liquid culture using the Qiagen DNeasy Blood and Tissue Microbial Kit following the manufacturers protocol for Gram-positive bacteria. DNA was sent to SeqCoast Genomics LLC (Portsmouth, NH USA) for library preparation and sequencing. Samples were prepared for whole genome sequencing using an Illumina DNA Prep tagmentation kit and unique dual indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300-cycle flow cell kit, producing 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling. Read demultiplexing, read trimming, and run analytics were performed using DRAGEN v3.10.12.

Submitted by: Loyola University Chicago

Study: Female Urinary Microbiota Genome Sequencing

PRJNA316969 • SRP072679 • All experiments • All runs

show Abstracthide Abstract

Recently, bacterial communities (microbiota) have been discovered in the female bladder. Thus, the “sterile urine” paradigm is no longer valid. In an effort to provide a comprehensive view of the newly discovered female urinary microbiota (FUM), we have established an Enhanced Quantitative Urine Culture (EQUC) protocol. EQUC isolates bacteria from 75-90% of urine samples deemed 'no growth' by the standard clinical microbiology urine culture method. We have begun to sequence and annotate the genomes of these isolated bacteria.

Sample: Lactobacillus crispatus UMB4277

SAMN41062563 • SRS21107989 • All experiments • All runs

Organism: Lactobacillus crispatus

Library:

Name: UMB4277

Instrument: NextSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 1.9M spots, 564.3M bases, 199.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR28784456	1,907,018	564.3M	199.2Mb	2024-04-23

ID:: 32663661

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